Details of experiments between the fragments P57105~6-103 and Q8TDW7~4548-4557
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SYNJ2BP
Protein A fragment: 6-103
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: FAT3
Protein B fragment: 4548-4557
Protein B site: PBM
Protein B sequence: FVETQHQTQV
Protein B construct: biotin-ttds-FVETQHQTQV
Average holdup BI: 0.14
Normalized holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.08