Details of experiments between the fragments P57105~6-103 and P50479~321-330
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SYNJ2BP
Protein A fragment: 6-103
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PDLIM4
Protein B fragment: 321-330
Protein B site: PBM
Protein B sequence: PSASCAVTAA
Protein B construct: Biotin-Ahx-PSASCAVTAA
Average holdup BI: -0.05
Holdup BI standard deviation: 0
Normalized holdup BI: -0.07
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.