Details of experiments between the fragments P57105~6-103 and O60759~350-359
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SYNJ2BP
Protein A fragment: 6-103
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PSCDBP
Protein B fragment: 350-359
Protein B site: PBM
Protein B sequence: RAVEEEESRF
Protein B construct: Biotin-Ahx-RAVEEEESRF
Average holdup BI: -0.09
Holdup BI standard deviation: 0.03
Normalized holdup BI: -0.11
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.