Details of experiments between P57105 and Q15599
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SYNJ2BP
Protein A fragment: 6-103
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: NHERF2
Protein B fragment: 328-337
Protein B site: PBM
Protein B sequence: NRKREIFSNF
Protein B construct: Biotin-Ahx-NRKREIFSNF
Average holdup BI: -0.04
Holdup BI standard deviation: 0.03
Normalized holdup BI: -0.05
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.