Details of experiments between the fragments P55345~24-96 and Q9UN86~294-308
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PRMT2
Protein A fragment: 24-96
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: G3BP2
Protein B fragment: 294-308
Protein B site: PxxP_2
Protein B sequence: QRPRERPGFPPRGPR
Protein B construct: Bio Ado Ado-QRPRERPGFPPRGPR-CONH2
Average holdup BI: -0.08
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
The affinity was below the detection threshold of the assay.