Details of experiments between the fragments P55345~24-96 and Q8IZ21~229-243
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PRMT2
Protein A fragment: 24-96
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PHACTR4
Protein B fragment: 229-243
Protein B site: PxxP_2
Protein B sequence: PAPRTLPAAPASTNT
Protein B construct: Bio Ado Ado-PAPRTLPAAPASTNT-CONH2
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.