Details of experiments between P53778 and Q9NZN5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAPK12
Protein A fragment: 358-367
Protein A site: PBM
Protein A sequence: GARVSKETPL
Protein A construct: biotin-ttds-GARVSKETPL
Protein B protein: ARHGEF12
Protein B fragment: 67-151
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Normalized holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.34
Experiment series 2
Protein A protein: MAPK12
Protein A fragment: 358-367
Protein A site: PBM
Protein A sequence: GARVSKETPL
Protein A construct: Biotin-ado-ado-GARVSKETPL
Protein B protein: ARHGEF12
Protein B fragment: 67-151
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Normalized holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.38