Details of experiments between P53778 and Q96QZ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAPK12
Protein A fragment: 358-367
Protein A site: PBM
Protein A sequence: GARVSKETPL
Protein A construct: biotin-ttds-GARVSKETPL
Protein B protein: MAGI1
Protein B fragment: 456-580
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.76
Experiment series 2
Protein A protein: MAPK12
Protein A fragment: 358-367
Protein A site: PBM
Protein A sequence: GARVSKETPL
Protein A construct: biotin-ttds-GARVSKETPL
Protein B protein: MAGI1
Protein B fragment: 1-106
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: MAPK12
Protein A fragment: 358-367
Protein A site: PBM
Protein A sequence: GARVSKETPL
Protein A construct: Biotin-ado-ado-GARVSKETPL
Protein B protein: MAGI1
Protein B fragment: 1-106
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: MAPK12
Protein A fragment: 358-367
Protein A site: PBM
Protein A sequence: GARVSKETPL
Protein A construct: Biotin-ado-ado-GARVSKETPL
Protein B protein: MAGI1
Protein B fragment: 456-580
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Normalized holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.06