Details of experiments between the fragments P53671~143-248 and Q14155~794-803
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LIMK2
Protein A fragment: 143-248
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGEF7
Protein B fragment: 794-803
Protein B site: PBM
Protein B sequence: NDPAWDETNL
Protein B construct: biotin-ttds-NDPAWDETNL
Average holdup BI: -0.11
Holdup BI standard deviation: 0.16
Normalized holdup BI: -0.11
Normalized holdup BI standard deviation: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.