Details of experiments between the fragments P53667~156-267 and Q9NS75~337-346
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LIMK1
Protein A fragment: 156-267
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CYSLTR2
Protein B fragment: 337-346
Protein B site: PBM
Protein B sequence: SVWLRKETRV
Protein B construct: biotin-ttds-SVWLRKETRV
Average holdup BI: -0.13
Immobilized partner concentration in holdup experiment (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.