Details of experiments between P50479 and Q8N448
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDLIM4
Protein A fragment: 237-246
Protein A site: PBM
Protein A modification: iso2
Protein A sequence: VYPNAKVELV
Protein A construct: biotin-ado-ado-VYPNAKVELV
Protein B protein: LNX2
Protein B fragment: 334-426
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.41
Normalized holdup BI: 0.41
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.62
Experiment series 2
Protein A protein: PDLIM4
Protein A fragment: 237-246
Protein A site: PBM
Protein A modification: iso2
Protein A sequence: VYPNAKVELV
Protein A construct: biotin-ado-ado-VYPNAKVELV
Protein B protein: LNX2
Protein B fragment: 459-560
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: PDLIM4
Protein A fragment: 321-330
Protein A site: PBM
Protein A sequence: PSASCAVTAA
Protein A construct: Biotin-Ahx-PSASCAVTAA
Protein B protein: LNX2
Protein B fragment: 334-426
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: PDLIM4
Protein A fragment: 321-330
Protein A site: PBM
Protein A sequence: PSASCAVTAA
Protein A construct: Biotin-Ahx-PSASCAVTAA
Protein B protein: LNX2
Protein B fragment: 459-560
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.