Details of experiments between the fragments P49418~615-695 and Q9UN86~294-308
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: G3BP2
Protein B fragment: 294-308
Protein B site: PxxP_2
Protein B sequence: QRPRERPGFPPRGPR
Protein B construct: Bio Ado Ado-QRPRERPGFPPRGPR-CONH2
Average holdup BI: 0.24
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
Measured pKd: 4.27