Details of experiments between the fragments P49418~615-695 and Q9BRD0~194-208
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: BUD13
Protein B fragment: 194-208
Protein B site: PxxP_1
Protein B sequence: RHDSPDPSPPRRPQH
Protein B construct: Biotinylated 15-mer peptide (crude)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 3.68