Details of experiments between the fragments P49418~615-695 and Q8IZ21~286-300
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PHACTR4
Protein B fragment: 286-300
Protein B site: PxxP_4
Protein B sequence: LLSKPSPPLPPKRGI
Protein B construct: Bio Ado Ado-LLSKPSPPLPPKRGI-CONH2
Average holdup BI: 0.37
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
Measured pKd: 4.56