Details of experiments between the fragments P49418~615-695 and Q5JSZ5~330-344
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRRC2B
Protein B fragment: 330-344
Protein B site: PxxP_3
Protein B sequence: RLGLSRPLRPLRQLV
Protein B construct: Bio Ado Ado-RLGLSRPLRPLRQLV-CONH2
Average holdup BI: 0.7
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 5.18