Details of experiments between P49418 and Q9ULL5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: avi(biotin)-his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1-2036
Protein B construct: endogenous protein (Jurkat)
Average holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.84
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.29.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 3))
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1215-1229
Protein B site: PxxP_1
Protein B sequence: GGTRLEPLKPLKIKL
Protein B construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Average holdup BI: -0.05
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 13-27
Protein B site: PxxP_2
Protein B modification: BAA86519.1
Protein B sequence: HPVRPPPPPPPPMPL
Protein B construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1300-1314
Protein B site: PxxP_3
Protein B sequence: QAGLTPPLSPPKSVP
Protein B construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1453-1467
Protein B site: PxxP_4
Protein B sequence: LEEKPPPTPPPAPTP
Protein B construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1664-1678
Protein B site: PxxP_5
Protein B sequence: RKPWHRPPVPVRRSG
Protein B construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Average holdup BI: 0.62
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 5.01
Experiment series 7
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1691-1705
Protein B site: PxxP_6
Protein B sequence: APPPKAPAPPPKPET
Protein B construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1697-1711
Protein B site: PxxP_7
Protein B sequence: PAPPPKPETPEKTTS
Protein B construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1720-1734
Protein B site: PxxP_8
Protein B sequence: ETAMPEPPAPEKPSL
Protein B construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1143-1157
Protein B site: PxxP_9
Protein B sequence: CPPNPGPDGPRRRGR
Protein B construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.54
Experiment series 11
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1161-1175
Protein B site: PxxP_10
Protein B sequence: KAKRDGPPRPRGRPR
Protein B construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Average holdup BI: 0.32
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.44
Experiment series 12
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 1309-1323
Protein B site: PxxP_11
Protein B sequence: PPKSVPPSVPARGLQ
Protein B construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Average holdup BI: 0.38
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.58
Experiment series 13
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 5-19
Protein B site: PxxP_12
Protein B modification: BAA86519.1
Protein B sequence: PGVPPGPPHPVRPPP
Protein B construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: AMPH
Protein A fragment: 615-695
Protein A site: SH3
Protein A construct: his6-MBP-TEVsite-SH3
Protein B protein: PRR12
Protein B fragment: 986-1000
Protein B site: PxxP_13
Protein B sequence: AYDPYGPYCPGRASG
Protein B construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.