Details of experiments between the fragments P48634~690-704 and P55345~24-96
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PRRC2A
Protein A fragment: 690-704
Protein A site: PxxP_15
Protein A sequence: VPAPQAPPPPPKALY
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: PRMT2
Protein B fragment: 24-96
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
Measured pKd: 3.7