Details of experiments between the fragments P36873~303-317 and P49418~615-695
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: PPP1CC
Protein A fragment: 303-317
Protein A site: PxxP_1
Protein A sequence: KPNATRPVTPPRGMI
Protein A construct: Bio Ado Ado-KPNATRPVTPPRGMI-CONH2

Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3

Average holdup BI: 0.38
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.57