Details of experiments between the fragments P36809~144-153 and Q86UL8~410-522
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 8.19
Standard deviation of dissociation constant (10-6M): 0.64
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 5.09
Experiment series 2
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.01
Experiment series 3
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.5
Normalized holdup BI: 0.55
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.04