Details of experiments between P36809 and Q9Y6N9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: USH1C
Protein B fragment: 75-178
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: USH1C
Protein B fragment: 208-299
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: USH1C
Protein B fragment: 441-548
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.