Details of experiments between P36809 and Q9NSN8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: SNTG1
Protein B fragment: 54-143
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.38
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.38
Experiment series 2
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: SNTG1
Protein B fragment: 54-143
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.41
Normalized holdup BI: 0.5
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.94