Details of experiments between P36809 and Q86UL8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 8.19
Standard deviation of dissociation constant (10-6M): 0.64
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 5.09
Experiment series 2
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.01
Experiment series 3
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 1141-1229
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.48
Experiment series 4
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 1-102
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 600-682
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 772-865
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 915-1017
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 410-522
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.5
Normalized holdup BI: 0.55
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.04
Experiment series 9
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 600-682
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.26
Normalized holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.57
Experiment series 10
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 915-1017
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Normalized holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.31
Experiment series 11
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 1141-1229
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.19
Experiment series 12
Protein A protein: HPV30-E6
Protein A fragment: 144-153
Protein A site: PBM
Protein A sequence: RHTTSTETAV
Protein A construct: biotin-ttds-RHTTSTETAV
Protein B protein: MAGI2
Protein B fragment: 772-865
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Normalized holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.07