Details of experiments between P36762 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A protein: HPV49-L2
Protein A fragment: 512-521
Protein A site: PBM
Protein A sequence: RRRKRKRTYL
Protein A construct: Biotin-Ahx-RRRKRKRTYL

Protein B protein: NHERF3
Protein B fragment: 2-107
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.49
Normalized holdup BI: 0.53
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.86

Experiment series 2

Protein A protein: HPV49-L2
Protein A fragment: 512-521
Protein A site: PBM
Protein A sequence: RRRKRKRTYL
Protein A construct: Biotin-Ahx-RRRKRKRTYL

Protein B protein: NHERF3
Protein B fragment: 238-329
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.2
Normalized holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.18

Experiment series 3

Protein A protein: HPV49-L2
Protein A fragment: 512-521
Protein A site: PBM
Protein A sequence: RRRKRKRTYL
Protein A construct: Biotin-Ahx-RRRKRKRTYL

Protein B protein: NHERF3
Protein B fragment: 375-459
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.15
Normalized holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4