Details of experiments between P36762 and O75970
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV49-L2
Protein A fragment: 512-521
Protein A site: PBM
Protein A sequence: RRRKRKRTYL
Protein A construct: Biotin-Ahx-RRRKRKRTYL
Protein B protein: MPDZ
Protein B fragment: 1624-1716
Protein B site: PDZ_10
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.94
Experiment series 2
Protein A protein: HPV49-L2
Protein A fragment: 512-521
Protein A site: PBM
Protein A sequence: RRRKRKRTYL
Protein A construct: Biotin-Ahx-RRRKRKRTYL
Protein B protein: MPDZ
Protein B fragment: 1858-1949
Protein B site: PDZ_12
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: HPV49-L2
Protein A fragment: 512-521
Protein A site: PBM
Protein A sequence: RRRKRKRTYL
Protein A construct: Biotin-Ahx-RRRKRKRTYL
Protein B protein: MPDZ
Protein B fragment: 688-793
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.