Details of experiments between the fragments P31946~1-246 and O43166~203-212
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SIPA1L1
Protein B fragment: 203-212
Protein B site: 14-3-3_pSer208
Protein B modification: (SEP)208
Protein B sequence: HREYGSTSSI
Protein B construct: HREYG(pSer)TSSI (crude synthetic peptide)
Measured dissociation constant (10-6M): <100
Immobilized partner concentration in holdup experiment (10-6M): 102
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 1
Measured pKd: 4