Details of experiments between the fragments P31946~1-246 and O43166~169-178
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SIPA1L1
Protein B fragment: 169-178
Protein B site: 14-3-3_pSer174
Protein B modification: (SEP)174
Protein B sequence: RIRQRSNSDI
Protein B construct: RIRQR(pSer)NSDI (crude synthetic peptide)
Measured dissociation constant (10-6M): <100
Immobilized partner concentration in holdup experiment (10-6M): 134
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 1
Measured pKd: 4