Details of experiments between P31946 and Q8TF72
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 414-423
Protein B site: 14-3-3_pSer419
Protein B modification: (SEP)419
Protein B sequence: RPQANSLGSL
Protein B construct: RPQAN(pSer)LGSL (crude synthetic peptide)
Average holdup BI: 0.68
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 7.86
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.32
Experiment series 2
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 414-423
Protein B site: 14-3-3_pSer419
Protein B modification: (SEP)419
Protein B sequence: RPQANSLGSL
Protein B construct: RPQAN(pSer)LGSL (crude synthetic peptide)
Average holdup BI: 0.68
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 7.76
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 4.2
Experiment series 3
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 618-627
Protein B site: 14-3-3_pSer623
Protein B modification: (SEP)623
Protein B sequence: RSSRLSEPWE
Protein B construct: RSSRL(pSer)EPWE (crude synthetic peptide)
Average holdup BI: 0.56
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 7.73
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.98
Experiment series 4
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 618-627
Protein B site: 14-3-3_pSer623
Protein B modification: (SEP)623
Protein B sequence: RSSRLSEPWE
Protein B construct: RSSRL(pSer)EPWE (crude synthetic peptide)
Average holdup BI: 0.54
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 7.38
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.06
Experiment series 5
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1457-1466
Protein B site: 14-3-3_pSer1462
Protein B modification: (SEP)1462
Protein B sequence: YQKQQSLPSL
Protein B construct: YQKQQ(pSer)LPSL (crude synthetic peptide)
Average holdup BI: 0.79
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.6
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.58
Experiment series 6
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1216-1225
Protein B site: 14-3-3_pSer1221
Protein B modification: (SEP)1221
Protein B sequence: AGKSMSAEDL
Protein B construct: AGKSM(pSer)AEDL (crude synthetic peptide)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.97
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.7
Experiment series 7
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1416-1425
Protein B site: 14-3-3_pSer1421
Protein B modification: (SEP)1421
Protein B sequence: TRKRVSLPQW
Protein B construct: TRKRV(pSer)LPQW (crude synthetic peptide)
Average holdup BI: 0.85
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.73
Experiment series 8
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1216-1225
Protein B site: 14-3-3_pSer1221
Protein B modification: (SEP)1221
Protein B sequence: AGKSMSAEDL
Protein B construct: AGKSM(pSer)AEDL (crude synthetic peptide)
Average holdup BI: 0.44
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 3.88
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.77
Experiment series 9
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1436-1445
Protein B site: 14-3-3_pSer1441
Protein B modification: (SEP)1441
Protein B sequence: AAREDSLPEE
Protein B construct: AARED(pSer)LPEE (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.43
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1436-1445
Protein B site: 14-3-3_pSer1441
Protein B modification: (SEP)1441
Protein B sequence: AAREDSLPEE
Protein B construct: AARED(pSer)LPEE (crude synthetic peptide)
Average holdup BI: -0.12
Holdup BI standard deviation: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 716-725
Protein B site: 14-3-3_pSer721
Protein B modification: (SEP)721
Protein B sequence: LDRQVSYPRP
Protein B construct: LDRQV(pSer)YPRP (crude synthetic peptide)
Average holdup BI: 0.18
Holdup BI standard deviation: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 107-116
Protein B site: 14-3-3_pThr112
Protein B modification: (TPO)112
Protein B sequence: RRDVCTDPGH
Protein B construct: RRDVC(pThr)DPGH (crude synthetic peptide)
Average holdup BI: -0.06
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 107-116
Protein B site: 14-3-3_pThr112
Protein B modification: (TPO)112
Protein B sequence: RRDVCTDPGH
Protein B construct: RRDVC(pThr)DPGH (crude synthetic peptide)
Average holdup BI: -0.4
Holdup BI standard deviation: 0.62
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.89
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 949-958
Protein B site: 14-3-3_pSer954
Protein B modification: (SEP)954
Protein B sequence: GAPVASRSWR
Protein B construct: GAPVA(pSer)RSWR (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.65
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1457-1466
Protein B site: 14-3-3_pSer1462
Protein B modification: (SEP)1462
Protein B sequence: YQKQQSLPSL
Protein B construct: YQKQQ(pSer)LPSL (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 16
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 949-958
Protein B site: 14-3-3_pSer954
Protein B modification: (SEP)954
Protein B sequence: GAPVASRSWR
Protein B construct: GAPVA(pSer)RSWR (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 17
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1016-1025
Protein B site: 14-3-3_pSer1021
Protein B modification: (SEP)1021
Protein B sequence: KKRSYSEPEK
Protein B construct: KKRSY(pSer)EPEK (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 102
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 18
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 1016-1025
Protein B site: 14-3-3_pSer1021
Protein B modification: (SEP)1021
Protein B sequence: KKRSYSEPEK
Protein B construct: KKRSY(pSer)EPEK (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 19
Protein A protein: YWHAB
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3beta
Protein B protein: SHROOM3
Protein B fragment: 716-725
Protein B site: 14-3-3_pSer721
Protein B modification: (SEP)721
Protein B sequence: LDRQVSYPRP
Protein B construct: LDRQV(pSer)YPRP (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.