Details of experiments between the fragments P30291~55-69 and P49418~615-695
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: WEE1
Protein A fragment: 55-69
Protein A site: PxxP_2
Protein A sequence: FQEPDSPLPPARSPT
Protein A construct: Bio Ado Ado-FQEPDSPLPPARSPT-CONH2
Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.76