Details of experiments between P29475 and Q81964
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NOS1
Protein A fragment: 9-101
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV59-E6
Protein B fragment: 151-160
Protein B site: PBM
Protein B sequence: QRQARSETLV
Protein B construct: biotin-ttds-QRQARSETLV
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: NOS1
Protein A fragment: 9-101
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV59-E6
Protein B fragment: 151-160
Protein B site: PBM
Protein B sequence: QRQARSETLV
Protein B construct: biotin-ttds-QRQARSETLV
Average holdup BI: 0.19
Normalized holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.26