Details of experiments between P27816 and Q8TBB1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAP4
Protein A fragment: 1143-1152
Protein A site: PBM
Protein A sequence: LDSQIQETSI
Protein A construct: biotin-ttds-LDSQIQETSI
Protein B protein: LNX1
Protein B fragment: 261-371
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: MAP4
Protein A fragment: 1143-1152
Protein A site: PBM
Protein A sequence: LDSQIQETSI
Protein A construct: biotin-ttds-LDSQIQETSI
Protein B protein: LNX1
Protein B fragment: 371-470
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.09
Normalized holdup BI: -0.1
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: MAP4
Protein A fragment: 1143-1152
Protein A site: PBM
Protein A sequence: LDSQIQETSI
Protein A construct: biotin-ttds-LDSQIQETSI
Protein B protein: LNX1
Protein B fragment: 500-596
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.