Details of experiments between P27348 and Q12802
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 2462-2471
Protein B site: 14-3-3_pThr2467
Protein B modification: (TPO)2467
Protein B sequence: PRRAETFGGF
Protein B construct: PRRAE(pThr)FGGF (crude synthetic peptide)
Average holdup BI: 0.97
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 7.38
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 5.35
Experiment series 2
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1924-1933
Protein B site: 14-3-3_pSer1929
Protein B modification: (SEP)1929
Protein B sequence: KFLSHSTDSL
Protein B construct: KFLSH(pSer)TDSL (crude synthetic peptide)
Average holdup BI: 0.49
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.49
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.86
Experiment series 3
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 2493-2502
Protein B site: 14-3-3_pSer2498
Protein B modification: (SEP)2498
Protein B sequence: LRRTESDSGL
Protein B construct: LRRTE(pSer)DSGL (crude synthetic peptide)
Average holdup BI: 0.3
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.52
Experiment series 4
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 2406-2415
Protein B site: 14-3-3_pThr2411
Protein B modification: (TPO)2411
Protein B sequence: LFRSNTEEAL
Protein B construct: LFRSN(pThr)EEAL (crude synthetic peptide)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.46
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1502-1511
Protein B site: 14-3-3_pSer1507
Protein B modification: (SEP)1507
Protein B sequence: SRDRQSLDGF
Protein B construct: SRDRQ(pSer)LDGF (crude synthetic peptide)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.29
Experiment series 6
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1745-1754
Protein B site: 14-3-3_pSer1750
Protein B modification: (SEP)1750
Protein B sequence: VSRTFSYIKN
Protein B construct: VSRTF(pSer)YIKN (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 3.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1871-1880
Protein B site: 14-3-3_pSer1876
Protein B modification: (SEP)1876
Protein B sequence: KERPRSAVLL
Protein B construct: KERPR(pSer)AVLL (crude synthetic peptide)
Average holdup BI: 0.41
Holdup BI standard deviation: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.72
Experiment series 8
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 2562-2571
Protein B site: 14-3-3_pSer2567
Protein B modification: (SEP)2567
Protein B sequence: LSRPSSLIEQ
Protein B construct: LSRPS(pSer)LIEQ (crude synthetic peptide)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1221-1230
Protein B site: 14-3-3_pSer1226
Protein B modification: (SEP)1226
Protein B sequence: SGRERSTPSL
Protein B construct: SGRER(pSer)TPSL (crude synthetic peptide)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.39
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1640-1649
Protein B site: 14-3-3_pSer1645
Protein B modification: (SEP)1645
Protein B sequence: VDSLVSLSEE
Protein B construct: VDSLV(pSer)LSEE (crude synthetic peptide)
Average holdup BI: -0.19
Holdup BI standard deviation: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1597-1606
Protein B site: 14-3-3_pSer1602
Protein B modification: (SEP)1602
Protein B sequence: HRRSFSLEGL
Protein B construct: HRRSF(pSer)LEGL (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.38
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1637-1646
Protein B site: 14-3-3_pSer1642
Protein B modification: (SEP)1642
Protein B sequence: EERVDSLVSL
Protein B construct: EERVD(pSer)LVSL (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.22
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 13
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 2468-2477
Protein B site: 14-3-3_pSer2473
Protein B modification: (SEP)2473
Protein B sequence: FGGFDSHQMN
Protein B construct: FGGFD(pSer)HQMN (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 1890-1899
Protein B site: 14-3-3_pSer1895
Protein B modification: (SEP)1895
Protein B sequence: FANRRSQQSV
Protein B construct: FANRR(pSer)QQSV (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: YWHAQ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3tau
Protein B protein: AKAP13
Protein B fragment: 2406-2415
Protein B site: 14-3-3_pThr2411
Protein B modification: (TPO)2411
Protein B sequence: LFRSNTEEAL
Protein B construct: biotin-ttds-LFRSN(pThr)EEAL (HPLC-purified synthetic peptide)
Measured dissociation constant (10-6M): 528
Standard deviation of dissociation constant (10-6M): 48
Experiment method: competitive Fluorescence Polarization
Details of affinity fitting: Competitive binding equation in ProFit
Date of measurement: 2022.02.08
Number of measurements: 3
Measured pKd: 3.28