Details of experiments between P27228 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV35-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SKPTRRETEV
Protein A construct: biotin-ttds-SKPTRRETEV
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.62
Immobilized partner concentration in holdup experiment (10-6M): 39.7
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.64
Experiment series 2
Protein A protein: HPV35-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SKPTRRETEV
Protein A construct: biotin-ttds-SKPTRRETEV
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 26.8
Standard deviation of dissociation constant (10-6M): 1.73
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.57
Experiment series 3
Protein A protein: HPV35-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A modification: (TPO)147
Protein A sequence: SKPTRRETEV
Protein A construct: Biotin-ttds-SKPTRREpTEV
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.59
Experiment series 4
Protein A protein: HPV35-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SKPTRRETEV
Protein A construct: biotin-ttds-SKPTRRETEV
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.44
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.44
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18.55
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 6
Measured pKd: 4.66
Experiment series 5
Protein A protein: HPV35-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SKPTRRETEV
Protein A construct: biotin-ttds-SKPTRRETEV
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.4
Normalized holdup BI: 0.46
Immobilized partner concentration in holdup experiment (10-6M): 18.55
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 4.7
Experiment series 6
Protein A protein: HPV35-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SKPTRRETEV
Protein A construct: biotin-ttds-SKPTRRETEV
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.37
Normalized holdup BI: 0.39
Immobilized partner concentration in holdup experiment (10-6M): 18.55
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 4.57
Experiment series 7
Protein A protein: HPV35-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SKPTRRETEV
Protein A construct: biotin-ttds-SKPTRRETEV
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.5
Normalized holdup BI: 0.5
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.8