Details of experiments between the fragments P27037~504-513 and Q14160~718-814
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ACVR2A
Protein A fragment: 504-513
Protein A site: PBM
Protein A sequence: VDFPPKESSL
Protein A construct: biotin-ttds-VDFPPKESSL
Protein B protein: SCRIB
Protein B fragment: 718-814
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 85.41
Standard deviation of dissociation constant (10-6M): 2.82
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.07
Experiment series 2
Protein A protein: ACVR2A
Protein A fragment: 504-513
Protein A site: PBM
Protein A sequence: VDFPPKESSL
Protein A construct: biotin-ttds-VDFPPKESSL
Protein B protein: SCRIB
Protein B fragment: 718-814
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.18
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 3.83