Details of experiments between P27037 and Q14CM0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ACVR2A
Protein A fragment: 504-513
Protein A site: PBM
Protein A sequence: VDFPPKESSL
Protein A construct: biotin-ttds-VDFPPKESSL
Protein B protein: FRMPD4
Protein B fragment: 67-160
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.