Details of experiments between P27037 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A protein: ACVR2A
Protein A fragment: 504-513
Protein A site: PBM
Protein A sequence: VDFPPKESSL
Protein A construct: biotin-ttds-VDFPPKESSL

Protein B protein: DLG1
Protein B fragment: 314-412
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.32
Normalized holdup BI: 0.36
Immobilized partner concentration in holdup experiment (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.24

Experiment series 2

Protein A protein: ACVR2A
Protein A fragment: 504-513
Protein A site: PBM
Protein A sequence: VDFPPKESSL
Protein A construct: biotin-ttds-VDFPPKESSL

Protein B protein: DLG1
Protein B fragment: 457-570
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.24
Normalized holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.11

Experiment series 3

Protein A protein: ACVR2A
Protein A fragment: 504-513
Protein A site: PBM
Protein A sequence: VDFPPKESSL
Protein A construct: biotin-ttds-VDFPPKESSL

Protein B protein: DLG1
Protein B fragment: 221-314
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.12
Normalized holdup BI: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.71