Details of experiments between the fragments P26554~142-151 and Q96RT1~1314-1412
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: LAP2
Protein B fragment: 1314-1412
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.75
Experiment series 2
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: LAP2
Protein B fragment: 1314-1412
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.69