Details of experiments between P26554 and Q9P202
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DFNB31
Protein B fragment: 136-226
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.44
Experiment series 2
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DFNB31
Protein B fragment: 263-379
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DFNB31
Protein B fragment: 136-226
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.47
Experiment series 4
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DFNB31
Protein B fragment: 263-379
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DFNB31
Protein B fragment: 813-904
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.