Details of experiments between P26554 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DLG2
Protein B fragment: 93-186
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.41
Immobilized partner concentration in holdup experiment (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.06
Experiment series 2
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.38
Immobilized partner concentration in holdup experiment (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.99
Experiment series 3
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.68
Experiment series 4
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.64
Normalized holdup BI: 0.64
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.97
Experiment series 5
Protein A protein: HPV51-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: RTRQRNETQV
Protein A construct: biotin-ttds-RTRQRNETQV
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.52
Normalized holdup BI: 0.52
Immobilized partner concentration in holdup experiment (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.75