Details of experiments between the fragments P23634~1232-1241 and Q9NUP9~85-176
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ATP2B4
Protein A fragment: 1232-1241
Protein A site: PBM
Protein A sequence: SSLQSLETSV
Protein A construct: biotin-ttds-SSLQSLETSV
Protein B protein: LIN7C
Protein B fragment: 85-176
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.63
Experiment series 2
Protein A protein: ATP2B4
Protein A fragment: 1232-1241
Protein A site: PBM
Protein A sequence: SSLQSLETSV
Protein A construct: biotin-ttds-SSLQSLETSV
Protein B protein: LIN7C
Protein B fragment: 85-176
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.3
Normalized holdup BI: 0.5
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.8
Experiment series 3
Protein A protein: ATP2B4
Protein A fragment: 1232-1241
Protein A site: PBM
Protein A sequence: SSLQSLETSV
Protein A construct: biotin-ttds-SSLQSLETSV
Protein B protein: LIN7C
Protein B fragment: 85-176
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.43
Normalized holdup BI: 0.48
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.76