Details of experiments between the fragments P23634~1232-1241 and Q15700~93-186
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ATP2B4
Protein A fragment: 1232-1241
Protein A site: PBM
Protein A sequence: SSLQSLETSV
Protein A construct: biotin-ttds-SSLQSLETSV
Protein B protein: DLG2
Protein B fragment: 93-186
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.94