Details of experiments between the fragments P22460~604-613 and Q96L92~40-141
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KCNA5
Protein A fragment: 604-613
Protein A site: PBM
Protein A sequence: CLDTSRETDL
Protein A construct: biotin-ttds-CLDTSRETDL
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.43
Experiment series 2
Protein A protein: KCNA5
Protein A fragment: 604-613
Protein A site: PBM
Protein A sequence: CLDTSRETDL
Protein A construct: biotin-ttds-CLDTSRETDL
Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.32
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.32
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.44