Details of experiments between P22460 and Q8TEW0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KCNA5
Protein A fragment: 604-613
Protein A site: PBM
Protein A sequence: CLDTSRETDL
Protein A construct: biotin-ttds-CLDTSRETDL
Protein B protein: PARD3
Protein B fragment: 264-372
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: KCNA5
Protein A fragment: 604-613
Protein A site: PBM
Protein A sequence: CLDTSRETDL
Protein A construct: biotin-ttds-CLDTSRETDL
Protein B protein: PARD3
Protein B fragment: 454-550
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: KCNA5
Protein A fragment: 604-613
Protein A site: PBM
Protein A sequence: CLDTSRETDL
Protein A construct: biotin-ttds-CLDTSRETDL
Protein B protein: PARD3
Protein B fragment: 582-685
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: KCNA5
Protein A fragment: 604-613
Protein A site: PBM
Protein A sequence: CLDTSRETDL
Protein A construct: biotin-ttds-CLDTSRETDL
Protein B protein: PARD3
Protein B fragment: 582-685
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.48