Details of experiments between the fragments P22459~644-653 and Q9Y2H9~965-1056
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KCNA4
Protein A fragment: 644-653
Protein A site: PBM
Protein A sequence: SNAKAVETDV
Protein A construct: biotin-ttds-SNAKAVETDV
Protein B protein: MAST1
Protein B fragment: 965-1056
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.26
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.32
Experiment series 2
Protein A protein: KCNA4
Protein A fragment: 644-653
Protein A site: PBM
Protein A sequence: SNAKAVETDV
Protein A construct: Biotin-ado-ado-SNAKAVETDV
Protein B protein: MAST1
Protein B fragment: 965-1056
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Normalized holdup BI: 0.37
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.56