Details of experiments between P22459 and Q8TBB1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: KCNA4
Protein A fragment: 644-653
Protein A site: PBM
Protein A sequence: SNAKAVETDV
Protein A construct: biotin-ttds-SNAKAVETDV
Protein B protein: LNX1
Protein B fragment: 261-371
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.77
Experiment series 2
Protein A protein: KCNA4
Protein A fragment: 644-653
Protein A site: PBM
Protein A sequence: SNAKAVETDV
Protein A construct: biotin-ttds-SNAKAVETDV
Protein B protein: LNX1
Protein B fragment: 371-470
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Holdup BI standard deviation: 0.07
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: KCNA4
Protein A fragment: 644-653
Protein A site: PBM
Protein A sequence: SNAKAVETDV
Protein A construct: biotin-ttds-SNAKAVETDV
Protein B protein: LNX1
Protein B fragment: 500-596
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: KCNA4
Protein A fragment: 644-653
Protein A site: PBM
Protein A sequence: SNAKAVETDV
Protein A construct: Biotin-ado-ado-SNAKAVETDV
Protein B protein: LNX1
Protein B fragment: 371-470
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: KCNA4
Protein A fragment: 644-653
Protein A site: PBM
Protein A sequence: SNAKAVETDV
Protein A construct: Biotin-ado-ado-SNAKAVETDV
Protein B protein: LNX1
Protein B fragment: 500-596
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: KCNA4
Protein A fragment: 644-653
Protein A site: PBM
Protein A sequence: SNAKAVETDV
Protein A construct: Biotin-ado-ado-SNAKAVETDV
Protein B protein: LNX1
Protein B fragment: 261-371
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.87