Details of experiments between the fragments P22234~1-425 and Q96L92~1-541
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PAICS
Protein A fragment: 1-425
Protein A construct: endogenous protein (Jurkat)
Protein B protein: SNX27
Protein B fragment: 1-541
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SNX27
Average holdup BI: -0.16
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.17
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.27.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 6))
PUBMED ID of publication: 36542723
Number of measurements: 6
The affinity was below the detection threshold of the assay.