Details of experiments between the fragments P20810~379-393 and Q14155~178-251
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CAST
Protein A fragment: 379-393
Protein A site: PxxP_2
Protein A sequence: SECKEKPSKPTEKTE
Protein A construct: Bio Ado Ado-SECKEKPSKPTEKTE-CONH2
Protein B protein: ARHGEF7
Protein B fragment: 178-251
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.