Details of experiments between P17386 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV31-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRRPRTETQV
Protein A construct: biotin-ttds-SRRPRTETQV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.78
Holdup BI standard deviation: 0
Normalized holdup BI: 0.78
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.37
Experiment series 2
Protein A protein: HPV31-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRRPRTETQV
Protein A construct: biotin-ttds-SRRPRTETQV
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.66
Holdup BI standard deviation: 0
Normalized holdup BI: 0.66
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.1