Details of experiments between P13569 and Q86UT5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CFTR
Protein A fragment: 1471-1480
Protein A site: PBM
Protein A sequence: TEEEVQDTRL
Protein A construct: biotin-ttds-TEEEVQDTRL
Protein B protein: NHERF4
Protein B fragment: 106-208
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: CFTR
Protein A fragment: 1471-1480
Protein A site: PBM
Protein A sequence: TEEEVQDTRL
Protein A construct: biotin-ttds-TEEEVQDTRL
Protein B protein: NHERF4
Protein B fragment: 220-307
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: CFTR
Protein A fragment: 1471-1480
Protein A site: PBM
Protein A sequence: TEEEVQDTRL
Protein A construct: biotin-ttds-TEEEVQDTRL
Protein B protein: NHERF4
Protein B fragment: 464-550
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: CFTR
Protein A fragment: 1471-1480
Protein A site: PBM
Protein A sequence: TEEEVQDTRL
Protein A construct: Biotin-ado-ado-TEEEVQDTRL
Protein B protein: NHERF4
Protein B fragment: 220-307
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.14
Normalized holdup BI: -0.14
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: CFTR
Protein A fragment: 1471-1480
Protein A site: PBM
Protein A sequence: TEEEVQDTRL
Protein A construct: Biotin-ado-ado-TEEEVQDTRL
Protein B protein: NHERF4
Protein B fragment: 464-550
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: CFTR
Protein A fragment: 1471-1480
Protein A site: PBM
Protein A sequence: TEEEVQDTRL
Protein A construct: Biotin-ado-ado-TEEEVQDTRL
Protein B protein: NHERF4
Protein B fragment: 106-208
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.89