Details of experiments between P0DTC9 and Q9HD26
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: GOPC
Protein B fragment: 278-373
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.