Details of experiments between P0DTC9 and Q12923
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: PTPN13
Protein B fragment: 1085-1188
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: PTPN13
Protein B fragment: 1362-1455
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: PTPN13
Protein B fragment: 1495-1591
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Holdup BI standard deviation: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: PTPN13
Protein B fragment: 1779-1873
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: SARS-Cov2-N
Protein A fragment: 410-419
Protein A site: PBM
Protein A sequence: SMSSADSTQA
Protein A construct: Biotin-(PEG)3-QQSMSSADSTQA
Protein B protein: PTPN13
Protein B fragment: 1875-1973
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.